Faculty of Medicine University of Miyazaki

Applied Physiology

MASUGI MARUYAMA, M.D., Ph.D.

masugi@med.miyazaki-u.ac.jp

Summary

Our laboratory focuses on applied research on plasminogen activator system in cell migration and invasion, cancer cell microenvironment, dermal wound healing process and promotion; and Biomaterials -based therapeutics. We also study the impact of naturaceuticals on pathophysiological events, in both cellular and animal models.

Research Projects

  1. Role of microenvironment in cancer cell metabolism using animal models.
  2. MicroRNA targeted therapeutics in relation to chronic non-healing wounds.
  3. Biomaterials based drug delivery system.
  4. Nano-naturaceuticals in wound and osteoporosis management.

Lab techniques

  1. Animal Cell culture
  2. Molecular Biology techniques including PCR, Cell cycle analysis, Ligation, Transformation,  Transfection, EMSA, ChIP, RNA interference
  3. General protein techniques including Western blotting, Immunoprecipitation, Zymography, Fibrinolysis, ELISA, Immunofluorescence
  4. Preparation of biomaterial-collagen scaffold systems for dermal substitutes

International collaboration

  1. Department of Haematology, Prince of Songkla University, Thailand
  2. Indian Institute of Technology, Kanpur, India
  3. Indian Council of Medical Research, Bhopal, India

Recent Publications

  1. NFkappaB activation is essential for miR-21 induction by TGFb1 in high glucose conditions. Madhyastha R., et al., BBRC 2014 (in press)
  2. Regional differences of microglial accumulation within 72 hours of hypoxia-ischemia and the effect of acetylcholine receptor agonist on brain damage and microglial activation in new-born rats. Furukawa S., et al., Brain Research 2014 (in press)
  3. MicroRNA signature in diabetic wound healing: promotive role of miR-21 in fibroblast migration. Madhyastha R., et al., International Wound Journal 2012; 9: 355-361.