論文
- Methodological considerations of the acetaminophen Detection Kit®: Involvement of molecular oxygen O2 in an indophenol reaction 査読あり
Shinkawa N., Marukawa M., Wada K., Yukawa N.
Legal Medicine 64 2023年9月
- Diversity in Infection Specificity between the Bloom-forming Microalga Heterosigma akashiwo and Its dsDNA Virus, Heterosigma akashiwo Virus 査読あり
Yusaku Funaoka, Haruna Hiromoto, Daichi Morimoto, Michiko Takahashi, Kei Wada, and Keizo Nagasaki
Microbes Environ. 38 ( 2 ) ME23036 - ME23036 2023年6月 - Neutron crystallography and quantum chemical analysis of bilin reductase PcyA mutants reveal substrate and catalytic residue protonation states 査読あり 国際共著
Tatsuya Joutsuka, Ryota Nanasawa, Keisuke Igarashi, Kazuki Horie, Masakazu Sugishima, Yoshinori Hagiwara, Kei Wada, Keiichi Fukuyama, Naomine Yano, Seiji Mori, Andreas Ostermann, Katsuhiro Kusaka, and Masaki Unno
J. Biol. Chem. 299 ( 1 ) 102763 2023年1月 - Degenerate PCR Targeting the Major Capsid Protein Gene of HcRNAV and Related Viruses 査読あり
Michiko Takahashi, Kei Wada, Syun-ichi Urayama, Yuichi Masuda, Keizo Nagasaki
Microbes and Environments 37 ( 5 ) ME21075 - ME21075 2022年4月 - Domain movements of NADPH–cytochrome P450 oxidoreductase (CPR) are required for the smooth electron transfer from CPR to heme–heme oxygenase-1 (HO-1) complex 査読あり
M. Sugishima, J. Taira, M. Iijima, H. Sato, K. Wada, M. Noguchi, K. Fukuyama, M. Takano, H. Sakamoto and K. Yamamoto
Acta Crystallographica Section A: Foundations and Advances A77 C1130 - C1130 2021年8月 - Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE 査読あり
Takayuki Nagae, Masashi Unno, Taiki Koizumi, Yohei Miyanoiri, Tomotsumi Fujisawa, Kento Masui, Takanari Kamo, Kei Wada, Toshihiko Eki, Yutaka Itoc, Yuu Hirosee,, and Masaki Mishima
Proceedings of the National Academy of Sciences of the United States of America ( 118 ) 2021年5月 - Crystal structures of hydroxymethylbilane synthase complexed with a substrate analog: a single substrate-binding site for four consecutive condensation step 査読あり
Sato H., Sugishima M., Tsukaguchi M., Masuko T., Iijima M., Takano M., Omata Y., Hirabayashi K., Wada K., Hisaeda Y., Yamamoto K.
Biochem. J. 478 1023 - 1042 2021年3月 - Pathological Features of Ruptured Coronary Plaque and Thrombus Interfaces: Fibrin and von Willebrand Factor as Platelet Scaffolds on Rupture Sites 査読あり
Atsushi Yamashita, Kensaku Nishihira, Toshihiro Gi, Kazunari Maekawa, Kinta Hatakeyama, Saki Horiuchi, Kei Wada, Yoshisato Shibata, Yujiro Asada
Thromb Haemost 121 ( 2 ) 234 - 241 2021年2月 - Chronological distribution of dinoflagellate-infecting RNA virus in marine sediment core 査読あり
MichikoTakahashi, Kei Wada, Yoshihito Takano, Kyouhei Matsuno, Yuichi Masuda, Kazuno Arai, Masafumi Murayama, Yuji Tomaru, Kouki Tanaka, Keizo Nagasaki
Science of The Total Environment ( 770 ) 145220 - 145228 2021年1月 - Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron‐sulfur cluster biosynthesis 査読あり
Sakiko Sato Yumeka Matsushima Miaki Kanazawa Naoyuki Tanaka Takashi Fujishiro Kouhei Kunichika Ryosuke Nakamura Hiroaki Tomioka Kei Wada Yasuhiro Takahashi
Molecular Microbiology 1 - 12 2020年11月 - Crystal structure of phytochromobilin synthase in complex with biliverdin IXα, a key enzyme in the biosynthesis of phytochrome 査読あり
Masakzu Sugishima, Kei Wada, Keiichi Fukuyama and Ken Yamamoto
Journal of Biological Chemistry 3 ( 295 ) 771 - 782 2020年1月 - Bilin-metabolizing enzymes: site-specific reductions catalyzed by two different type of enzymes 招待あり 査読あり
Masakazu Sugishima, KeiWada, Masaki Unno, Keiichi Fukuyama
Current Opinion in Structural Biology ( 59 ) 73 - 80 2019年12月 - Super-activator variants of the cyanobacterial transcriptional regulator ChlR essential for tetrapyrrole biosynthesis under low oxygen conditions 査読あり
Yuto Hiraide, Haruki Yamamoto, Yasushi Kawajiri, Hisanori Yamakawa, Kei Wada & Yuichi Fujita
Bioscience, Biotechnology, and Biochemistry ( 84 ) 481 - 490 2019年11月 - Identification of IscU residues critical for de novo iron‐sulfur cluster assembly 査読あり
Naoyuki Tanaka, Eiki Yuda, Takashi Fujishiro, Kei Hirabayashi, Kei Wada, Yasuhiro Takahashi
Molecular Microbiology 6 ( 112 ) 2019年9月 - Crystal structure of a NADPH‐cytochrome P450 oxidoreductase (CYPOR) and heme oxygenase 1 fusion protein implies a conformational change in CYPOR upon NADPH/NADP+ binding 査読あり
Masakazu Sugishima, Hideki Sato, Kei Wada, Kei Wada, Ken Yamamoto
FEBS Letters 2019年3月 - Recent Advances in the Understanding of the Reaction Chemistries of the Heme Catabolizing Enzymes HO and BVR Based on High Resolution Protein Structures 査読あり
Sugishima M, Wada K, Fukuyama K.
Current Medicinal Chemistry 2018年12月 - Crystal Growth of a Bilin Reductase PcyA I86D Mutant-Substrate Complex for Neutron Crystallography 査読あり
Keisuke Igarashi, Yoshinori Hagiwara, Masakazu Sugishima, Kei Wada, Keiichi Fukuyama, Atsushi Ikeda,Naomine Yano, Katsuhiro Kusaka, Andreas Ostermann, Masaki Unno
Crystal Growth & Design. 18 ( 9 ) 5181 - 5181 2018年7月 - Synthesis and Evaluation of the Inhibitory Activity of the Four Stereoisomers of the Potent and Selective Human γ-Glutamyl Transpeptidase Inhibitor GGsTop 査読あり
Bunta Watanabe, Yukiko Tabucchi, Kei Wada, Jun Hiratake
Bioorganic & Medicinal Chemistry Letters 24 ( 21 ) 5340 - 5352 2017年11月 - Mapping the key residues of SufB and SufD essential for biosynthesis of iron-sulfur clusters. 査読あり
Eiki Yuda, Naoyuki Tanaka, Takashi Fujishiro, Nao Yokoyama, Kei Hirabayashi, Keiichi Fukuyama, Kei Wada, Yasuhiro Takahashi
Scientific Reports 7 9387 2017年8月 - Structure-Dependent Binding of hnRNPA1 to Telomere RNA 査読あり
Liu X, Ishizuka T, Bao HL, Wada K, Takeda Y, Iida K, Nagasawa K, Yang D, Xu Y
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139 ( 22 ) 7533 - 7539 2017年5月 - 化学機械エンジン と呼ばれるタンパク質群 - ABCタンパク質がもつ機能性ドメインの多 査読あり
和田啓、高橋康弘
化学 2017年4月 - A substrate-bound structure of cyanobacterial biliverdin reductase identifies stacked substrates as critical for activity 査読あり
Takao H, Hirabayashi K, Nishigaya Y, Kouriki H, Nakaniwa T, Hagiwara Y, Harada J, Sato H, Yamazaki T, Sakakibara Y, Suiko M, Asada Y, Takahashi Y, Yamamoto K, Fukuyama K, Sugishima M, Wada K
Nature Communications 2017年2月 - Phosphonate-based irreversible inhibitors of human γ-glutamyl transpeptidase (GGT). GGsTop is a non-toxic and highly selective inhibitor with critical electrostatic interaction with an active-site residue Lys562 for enhanced inhibitory activityΔ
Kamiyama A,Nakajima M,Han L,Wada K,Mizutani M,Tabuchi Y,Kojima-Yuasa A,Matsui-Yuasa l,Suzuki H,Fukuyama K,Watanabe B,Hiratake J
Bioorganic & medicinal chemistry 1 ( 24 ) 5340 - 5352 2016年11月 - Atomic-resolution structure of the phycocyanobilin:ferredoxin oxidoreductase (PcyA) I86D mutant in complex with fully protonated biliverdin 査読あり
Hagiwara Y, Wada K, Irikawa T, Sato H, Unno M, Yamamoto K, Fukuyama K, Sugishima M
FEBS letter 590 ( 19 ) 3425 - 3434 2016年9月 - ビリン還元酵素PcyAと基質ビリベルジン複合体の中性子結晶構造解析で見えてきたもの
海野昌喜、日下勝弘、玉田太郎、杉島正一、和田啓、萩原義徳、福山恵一
日本中性子科学会誌「波紋」 2016年 - Neutron Crystallography of the Complex Between Photosynthetic Pigment of Phycocyanobilin Synthesizing Enzyme and Substrate Biliverdin IXα 招待あり 査読あり
福山 恵一，和田 啓，杉島 正一，海野 昌喜
生化学 87 ( 6 ) 753 - 757 2015年12月 - Functional Dynamics Revealed by the Structure of theSufBCD Complex, a Novel ATP-binding Cassette (ABC) Protein That Serves as a Scaffold for Iron-Sulfur Cluster Biogenesis 査読あり
Kei Hirabayashi, Eiki Yuda, Naoyuki Tanaka, Sumie Katayama, Kenji Iwasaki, Takashi Matsumoto, Genji Kurisu, F. Wayne Outten, Keiichi Fukuyama, Yasuhiro Takahashi, Kei Wada*
J. Biol. Chem. 290 ( 50 ) 29717 - 29731 2015年12月 - Two Protonation States and Structural Features of a Bilin Reductase PcyA Revealed by Neutron Crystallography 査読あり
海野昌喜、杉島正一、和田 啓、萩原義徳、日下勝弘、玉田太郎、福山恵一
日本結晶学会誌 57 ( 5 ) 297 - 303 2015年10月 - Insights into the Proton Transfer Mechanism of a Bilin Reductase PcyA Following Neutron Crystallography 査読あり
Unno M, Ishikawa-Suto K, Kusaka K, Tamada T, Hagiwara Y, Sugishima M, Wada K, Yamada T, Tomoyori K, Hosoya T, Tanaka, I, Niimura N, Kuroki R, Inaka K, Ishihara M, Fukuyama K.
Journal of the American Chemical Society 137 ( 16 ) 5452 - 5460 2015年4月 - Crystal structure of the catalase-peroxidase KatG W78F mutant from Synechococcus elongatus PCC7942 in complex with the antitubercular pro-drug isoniazid. 査読あり
S. Kamachi, K. Hirabayashi, M. Tamoi, S. Shigeoka, T. Tada*, K. Wada*.
FEBS Lett. 589 ( 1 ) 131 - 137 2015年1月 - The crystal structure of isoniazid-bound KatG catalase-peroxidase from Synechococcus elongatus PCC7942 査読あり
Kamachi S, Hirabayashi K, Tamoi M, Shigeoka S, Tada T, Wada K*.
FEBS Journal 282 ( 1 ) 54 - 64 2015年1月 - Structural basis for the electron transfer from an open form of NADPH-cytochrome P450 oxidoreductase to heme oxygenase. 査読あり
Sugishima M, Sato H, Higashimoto Y, Harada J, Wada K, Fukuyama K, Noguchi M.
Proceedings of the National Academy of Sciences 111 ( 7 ) 2524 - 2529 2014年2月 - Structure of Bacillus subtilis γ-glutamyltranspeptidase in complex with acivicin: diversity of the binding mode of a classical and electrophilic active-site-directed glutamate analogue 査読あり
Ida, T., Suzuki, H,, Fukuyama, K., Hiratake, J,, Wada, K.*
Acta Crystallographica Section D Biological Crystallography 70 607 - 614 2014年1月 - The 2.2 Å resolution structure of the catalase-peroxidase KatG from Synechococcus elongatus PCC7942. 査読あり
Kamachi, S., Wada, K.*, Tamoi, M., Shigeoka, S., Tada, T.
Acta Crystallogr.,Sect.F 70 288 - 293 2014年1月 - Cysteine 295 indirectly affects Ni coordination of carbon monoxide dehydrogenase-II C-cluster 査読あり
Inoue T, Takao K, Yoshida T, Wada K, Daifuku T, Yoneda Y, Fukuyama K, Sako Y.
Biochemical and Biophysical Research Communications 441 13 - 17 2013年11月 - Thermal denaturation and renaturation of γ-glutamyltranspeptidase of Escherichia coli. 査読あり
Ho TV, Kamei K, Wada K, Fukuyama K, Suzuki H.
Biosci. Biotechnol. Biochem. 77 409 - 412 2013年2月 - Establishment of a Bacterial Expression System andImmunoassay Platform for the Major Capsid Protein of HcRNAV, a Dinoflagellate- Infecting RNA Virus. Microbes Environ. 査読あり
Wada K, Kimura K, Hasegawa A, Fukuyama K, Nagasaki K.
Microbes Environ. 27 483 - 489 2012年10月 - A Simple and Large Scale Over-Expression Method for Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium Carboxydothermus hydrogenoformans 査読あり
Inoue, T., Yoshida, T., Wada, K., Daifuku, T., Fukuyama, K. and Sako, Y.
Biosci. Biotech. Biochem. 75 1392 - 1394 2011年7月 - Mapping of protein-protein interaction sites in the plant-type [2Fe-2S] ferredoxin. 査読あり
Kameda H, Hirabayashi K, Wada K, Fukuyama K.
PLoS ONE 6 e21947 2011年7月 - Expression, purification and preliminary X-ray crystallographic analysis of cyanobacterial biliverdin reductase 査読あり
Watanabe, A., Hirata, K., Hagiwara, Y., Yutani, Y., Sugishima, M., Yamamoto, M., Fukuyama, K., and Wada K.*
Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 67 313 - 317 2011年2月 - One residue substitution in PcyA leads to unexpected changes in tetrapyrrole substrate binding. 査読あり
Wada, K., Hagiwara, Y., Yutani, Y. and Fukuyama, K.
Biochem. Biophys. Res. Commun. 402 373 - 377 2010年11月 - Enhancement of glutaryl-7-aminocephalosporanic acid acylase activity of γ-glutamyltranspeptidase of Bacillus subtilis. 査読あり
Suzuki,H., Yamada, C., Kijima, K., Ishihara, S., Wada, K., Fukuyama, K., and Kumagai, H.
Biotechnol J. 5 829 - 837 2010年8月 - Crystal structure of the halotolerant γ-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals its unique architecture of the solvent-exposed catalytic pocket. 査読あり
Wada, K., Irie, M., Suzuki, H. and Fukuyama, K.
FEBS J. 277 1000 - 1009 2010年1月 - Molecular Dynamism of Fe-S Cluster Biosynthesis Implicated by the Structure of the SufC2-SufD2 Complex. 査読あり
Wada, K., Sumi, N., Nagai, R., Iwasaki, K., Sato, T., Suzuki, K., Hasegawa, Y., Kitaoka, S., Minami, Y., Outten, W., F., Takahashi, Y. and Fukuyama, K.
J. Mol. Biol., 387 245 - 258 2009年3月 - The asymmetric trimeric architecture of [2Fe-2S] IscU: Implications for its scaffolding during iron-sulfur cluster biosynthesis 査読あり
Shimomura, Y., Wada, K., Fukuyama, K. and Takahashi, Y.
J. Mol. Biol. 383 133 - 143 2008年10月 - Crystal Structures of Escherichia coli γ-Glutamyltranspeptidase in Complex with Azaserine and Acivicin: Novel Mechanistic Implication for Inhibition by Glutamine Antagonists 査読あり
Wada, K., Hiratake, J., Irie, M., Okada, T., Yamada, C., Kumagai, H., Suzuki, H. and Fukuyama, K.
J. Mol. Biol. 380 361 - 372 2008年6月 - Improvement of Glutaryl-7-aminocephalosporanic Acid Acylase Activity of Bacterial γ-Glutamyltranspeptidase 査読あり
Yamada, C., Kijima, K., Ishihara, S., Miwa, C., Wada, K., Okada, T., Fukuyama, K., Kumagai, H. and Suzuki, H.
Appl. Environ. Microbiol. 74 3400 - 3409 2008年4月 - Crystal Structures of CbiL, a Methyltransferase Involved in Anaerobic Vitamin B12 Biosynthesis, and its Complex with S-Adenosylhomocysteine: Implications for the Reaction Mechanism 査読あり
Wada, K.*, Harada, J., Yaeda, Y., Tamiaki, H., Oh-oka, H and Fukuyama., K.
FEBS Journal 274 563 - 573 2007年7月 - Crystal structure of the γ-glutamyltranspeptidase precursor protein from Escherichia coli. Structural changes upon autocatalytic processing and implications for the maturation mechanism. 査読あり
Okada, T., Suzuki, K., Wada, K., Kumagai, H and Fukuyama, K.
J. Biol. Chem. 288 2433 - 2439 2007年1月 - Structure of apo-glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC7942. 査読あり
Kitatani, T., Nakamura, Y., Wada, K., Kinoshita, T., Tamoi, M., Shigeoka, S. and Tada. T.
Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62 727 - 730 2006年8月 - Crystal structures of γ-glutamyltranspeptidase from Escherichia coli, a key enzyme in glutathione metabolism, and its reaction intermediate. 査読あり
Okada, T., Suzuki, H., Wada, K., Kumagai, H. and Fukuyama, K.
Proc. Natl. Acad. Sci. USA 103 6471 - 6476 2006年8月 - Crystal Structures of BchU, a Methyltransferase Involved in Bacteriochlorophyll c Biosynthesis, and its Complex with S-Adenosylhomocysteine: Implications for Reaction Mechanism. 査読あり
Wada, K., Yamaguchi, H., Harada, J., Niimi, K., Osumi, S., Saga, Y., Oh-oka, H., Tamiaki, H. and Fukuyama, K.
J. Mol. Biol. 360 839 - 849 2006年7月 - Structure of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC7942 complexed with NADP. 査読あり
Kitatani, T., Nakamura, Y., Wada, K., Kinoshita, T., Tamoi, M., Shigeoka, S. and Tada. T.
Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62 315 - 319 2006年4月 - Crystal structure of Escherichia coli SufC, an ABC-type ATPase component of the SUF iron-sulfur cluster assembly machinery. 査読あり
Kitaoka, S., Wada, K., Hasegawa, Y., Minami, Y., Fukuyama, K. and Takahashi, Y.
FEBS Lett. 580 137 - 143 2006年1月 - Crystal structure of Escherichia coli SufA involved in biosynthesis of iron-sulfur clusters: implications for a functional dimer. 査読あり
Wada, K., Hasegawa, Y., Gong, Z., Minami, Y., Fukuyama, K. and Takahashi, Y.
FEBS Lett. 579 6543 - 6548 2005年12月 - Identification of variant molecules of Bacillus thermoproteolyticus ferredoxin: crystal structure reveals bound coenzyme A and an unexpected [3Fe-4S] cluster associated with a canonical [4Fe-4S] ligand motif. 査読あり
Shirakawa, T., Takahashi, Y., Wada, K., Hirota, J., Takao, T., Ohmori, D. and Fukuyama, K.
Biochemistry 44 12402 - 12410 2005年9月 - Crystallization and preliminary X-ray diffraction study of BchU, a methyltransferase from Chlorobium tepidum involved in bacteriochlorophyll c biosynthesis. 査読あり
Harada, J., Wada, K., Yamaguchi, H., Oh-oka, H., Tamiaki, H. and Fukuyama, K.
Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun 61 712 - 714 2005年7月 - Structural basis for thermostability of endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3. 査読あり
Yamaguchi, A., Tada, T., Wada, K., Nakaniwa, T., Kitatani, T., Sogabe, Y., Takao, M., Sakai, T. and Nishimura, K.
J. Biochem 137 587 - 592 2005年5月 - Crystal structure of chloroplastic ascorbate peroxidase from tobacco plants and structural insights for its instability. 査読あり
Wada, K., Tada, T., Nakamura, Y., Ishikawa, T., Yabuta, Y., Yoshimura, K., Shigeoka, S. and Nishimura, K.
J. Biochem 134 239 - 244 2003年8月 - Crystallization and preliminary X-ray diffraction analysis of chloroplastic ascorbate peroxidase from tobacco plants. 査読あり
Wada, K., Tada, T., Nakamura, Y., Yoshimura, K., Shigeoka, S. and K. Nishimura.
Acta Crystallograph. Sect. D Struct. Biol. Cryst. Commun D58 559 - 561 2002年3月 - Crystallization and preliminary X-ray diffraction studies of catalase-peroxidase from Synechococcus PCC 7942. 査読あり
Wada, K., Tada, T., Nakamura, Y., Kinoshita, T., Tamoi, M., Shigeoka S. and Nishimura, K.
Acta Crystallograph. Sect. D Struct. Biol. Cryst. Commun D58 157 - 159 2002年1月 - Characterization of monoclonal antibodies against ascorbate peroxidase isoenzymes: purification and epitope-mapping using immunoaffinity column choromatography. 査読あり
Yoshimura, Y., Ishikawa, T., Wada, K., Takeda, T., Kamata, Y., Tada, T., Nishimura, K., Nakano, Y. and Shigeoka, S.
Biochim. Biophys. Acta, 1526 168 - 174 2001年5月 - Crystallization and preliminary X-ray diffraction analysis of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase of Synechococcus PCC 7942. 査読あり
Nakamura, Y., Tada, T., Wada, K., Kinoshita. T., Tamoi, M., Shigeoka, S. and Nishimura, K.
Acta Crystallograph. Sect. D Struct. Biol. Cryst. Commun D57 879 - 881 2001年3月 - Crystallization and preliminary X-ray analysis of a novel pectolytic enzyme, polymethoxygalacturonase SX1 from Trichosporon penicillatum. 査読あり
Tada, T., Lu, C, -T., Nakamura, Y., Wada, K., Katsuya, Y. and Nishimura, K.
Acta Crystallograph. Sect. D Struct. Biol. Cryst. Commun D57 457 - 458 2001年3月 - Purification, crystallization and preliminary X-ray diffraction analysis of the fructose-1,6-/sedoheptulose-1,7-bisphosphatase of Synechococcus PCC 7942. 査読あり
Nakamura, Y., Tada, T., Wada, K., Kinoshita, T., Tamoi, M., Shigeoka, S., and Nishimura, K.
Acta Crystallograph. Sect. D Struct. Biol. Cryst. Commun D57 454 - 456 2001年1月 - Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon, penicillatum. 査読あり
Lu, C, -T., Tada, T., Nakamura, Y., Wada, K., Nishimura, K., Katsuya, Y., Sawada, M. and Sakai, Y
Acta Crystallograph. Sect. D Struct. Biol. Cryst. Commun D56 1668 - 1669 2000年12月
