我们开发了一种基于高锰酸钾氧化反应的光学显微镜用石蜡切片电子染色法，可用于低真空扫描电子显微镜下的立体微观结构解析。

Development of a new a new metal staining method by potassium permanganate oxidation that allows uranium-free imaging of cell/tissue architectures in low-vacuum scanning electron microscopy

我们开发了一种突破性的“基于高锰酸钾氧化反应的电子染色法”，无需使用19世纪60年代开发的传统方法中所需的醋酸铀（国际管制物质），即可通过低真空扫描电子显微镜对光学显微镜用石蜡切片进行立体微观结构解析。
We have developed an epoch-making "uranium-free" metal staining method by potassium permanganate oxidation that allows uranium-free three-dimensional imaging of cell/tissue architectures in paraffin section by low-vacuum scanning electron microscopy

Sawaguchi, A. et al. npj Imaging 2, 40 (2024)
由日本科学技术振兴机构（JST）在《科学日本》介绍
由日本科学技术振兴机构（JST）在《客观日本》介绍

利用台式低真空扫描电子显微镜的优势和特性
开发出一种可视化生物物质定位的全新金纳米颗粒标记法

Development of a new gold nanoparticle labeling method for visualizing the localization of biological materials by utilizing the advantages and properties of desktop low-vacuum scanning electron microscopy

我们开发了一种新型金纳米颗粒标记法，该方法基于酶标抗体法免疫组化染色的石蜡切片，通过氯化金处理原位生长金纳米颗粒，从而轻松实现目标生物物质的定位可视化。
We have developed a new gold nanoparticle labeling method that allows easy visualization of the localization of target biological material by growing gold nanoparticles formed by treating with gold chloride on paraffin sections stained with immunohistochemistry using an enzyme-antibody method.

Sawaguchi, A. et al. Commun Biol 4, 710 (2021)

利用台式低真空扫描电子显微镜优势和特性的光学显微镜用
厚石蜡切片：高分辨率立体构建解析法的开发

Development of a high-resolution stereoscopic analysis method for thick paraffin sections by taking advantage of the advantages and characteristics of a desktop low-vacuum scanning electron microscope

我们开发了一种新型分析方法：充分发挥台式低真空扫描电镜的优势与特性，可对厚度为20-30微米的石蜡切片进行三维成像，捕捉构成生物体的组织与细胞结构，且该设备无需对切片进行抗荷电喷镀即可观察整张玻片。

We have developed a new analysis method that captures the tissues and cells that make up living organisms in three dimensions by taking advantage of the advantages and characteristics of a desktop low-vacuum scanning electron microscope, which can observe entire glass slides without applying antistatic treatment to paraffin sections sliced to a thickness of 20 to 30 μm.

Sawaguchi, A. et al. Sci Rep 8: 7479 (2018)

服务于iPS细胞来源血小板制剂开发与临床应用的电子显微镜解析
Electron Microscopical Evaluation of iPS Cell-Derived Human Blood Platelets for Clinical Transfusion

作为京都大学iPS细胞研究所(CiRA)江藤浩之教授实验室、Megakaryon株式会社以及日立高新技术株式会社(Hitachi High-Tech)的产学研合作项目，我们正在开展血小板及其前体细胞巨核细胞的超微形态分析，旨在能够早日实现iPS细胞来源血小板制剂的开发与临床应用。

As an industry-academia collaboration project with Prof. Koji Eto's lab at the Center for iPS Cell Research and Application (CiRA) in Kyoto University, Megakaryon Co., Ltd., and Hitachi High-Tech, Ltd., we are conducting ultra-micromorphological analysis of platelets and their progenitor cells, megakaryocytes, with a view to the early realization of iPS cell-derived platelet products and clinical applications.

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CLEM：光电关联显微技术 (Correlative Light and Electron Microscopy)
将光学显微镜筛选观察获得的最佳视野，定位至同一区域进行电镜观察！

在形态学分析中，建议先利用光学显微镜掌握观察对象的整体结构，然后再通过电子显微镜观察其细节。此外，在学术论文中，若能同时展示同一视野的光镜和电镜图像，将更具说服力。以下概述了光电关联显微技术（CLEM：Correlative Light and Electron Microscopy），即对甲苯胺蓝染色的超薄切片进行光镜观察与拍摄，再经树脂包埋后进行电镜观察。
In morphological analysis, it is recommended to first grasp the overall picture of the object to be observed at the light microscope level, and then observe the details at the electron microscope level. In addition, in academic papers, it will be more persuasive if electron microscope images with the same field of view can be presented alongside light microscopic images. This section provides an overview of Correlative Light and Electron Microscopy (CLEM), in which quasi-ultrathin sections stained with toluidine blue staining are observed and photographed by light microscopy, reembedded in resin, and electron microscopy is performed.

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