【 英文原著 】
クロイツフェルトーヤコブ病に関する脳病理組織所見
Shijo, M., Yoshimura, M., Omae, T., et al. Altered properties of amyloidogenic prion protein in genetic Creutzfeldt-Jakob disease with PRNP V180I mutation in response to pentosan polysulfate.
Brain Pathol. 33(5): e13197, 2023 Open Access
DOI: 10.1111/bpa.13197
前眼房炎症におけるZinn小帯の組織所見
Takahashi, A., Arima, T., Toda, E., et al. A Novel Multi-Observation System to Study the Effects of Anterior Ocular Inflammation in Zinn's Zonule Using One Specimen.
Int J Mol Sci. 24(7): 6254, 2023 Open Access
DOI: 10.3390/ijms24076254
凍結固定法による筋膜の超微形態観察
Imazato, H., Takahashi, N., Hirakawa, Y., et al. Three-dimensional fine structures in deep fascia revealed by combined use of cryo-fixed histochemistry and low-vacuum scanning microscopy.
Sci Rep. 13(1): 6352, 2023 Open Access
DOI: 10.1038/s41598-023-33479-3
腎糸球体基底膜及び足細胞の微細構造変化
Lan, P., Kang, D., Mii, A., et al. Evaluation of ultrastructural alterations of glomerular basement membrane and podocytes in glomeruli by low-vacuum scanning electron microscopy.
Clin Exp Nephrol. 26(3):216-225, 2022 Open Access
DOI: 10.1007/s10157-021-02147-z
DAB標識免疫染色の電子染色増感法
Arai, Y., Takeuchi, K., Hatanaka, S., et al. Heavy Metal Enhancement Technique for Diaminobenzidine in Immunohistochemistry Enables Ultrastructural Observation by Low-vacuum Scanning Electron Microscopy.
J Histochem Cytochem. 70(6): 427-436, 2022 Free PMC article
DOI: 10.1369/00221554221102996
紫色採尿バッグ症候群の解析事例
Abe, M., Furuichi, M., Ishimitsu, T., et al. Analysis of purple urine bag syndrome by low vacuum scanning electron microscopy.
Med Mol Morphol. 55(2):123-130, 2022 Open Access
DOI: 10.1007/s00795-022-00313-0
デスクトップ型低真空走査電子顕微鏡の利点・特性を活かした
生体物質の局在を可視化する新たな金ナノ粒子標識法の開発
In situ strategy for biomedical target localization via nanogold nucleation and secondary growth
酵素抗体法を用いて免疫組織化学染色された光顕用パラフィン切片に塩化金酸処理を施して形成された金ナノ粒子を成長させ、標的生体物質の局在を電子顕微鏡で容易に可視化する新たな金ナノ粒子標識法を開発しました。
新たな金ナノ粒子標識法の開発
Sawaguchi, A., Kamimura, T., Takahashi, N., et al. In situ strategy for biomedical target localization via nanogold nucleation and secondary growth.
Commun Biol. 4(1):710, 2021 Open Access
DOI: 10.1038/s42003-021-02246-3
ループス腎炎における病理組織所見
Yoshida, M. Hirashio, S., Doi, T., et al. Low-Vacuum Scanning Electron Microscopy to Assess Histopathological Resolution of Class V Lupus Nephritis: A Case Report.
Case Rep Nephrol Dial. 11(1): 36-47, 2021 Free PMC article
DOI: 10.1159/000509470
角膜創傷治癒と血管新生に関するパラフィン切片観察
Arima T., Uchiyama, M., Shimizu, A., et al. Observation of Corneal Wound Healing and Angiogenesis Using Low-Vacuum Scanning Electron Microscopy.
Transl Vis Sci Technol. 9(6): 14, 2020 Open Access
DOI: 10.1167/tvst.9.6.14
腎移植後の早期糸球体病変に関するパラフィン切片観察
Onishi, H., Oguchi, H., Shinoda, K., et al. Pathological Analysis of Early Transplant Glomerulopathy in Renal Allografts Using Low-Vacuum Scanning Electron Microscopy.
Nephron. 144 Suppl 1: 71-78, 2020 Free PMC article
DOI: 10.1159/000512136
ヒト腎移植後の抗体を介した拒絶反応の早期診断
Yokoyama, H., Okada, S., Yamada, Y., et al. Low-vacuum scanning electron microscopy may allow early diagnosis of human renal transplant antibody-mediated rejection.
Biomed Res. 41(2): 81-90, 2020 Free PMC article
DOI: 10.2220/biomedres.41.81
血管内皮glysocalyxのアルシアンブルー・銀増感染色
Mukai, S., Takaki, T., Nagumo, T., et al. Three-dimensional electron microscopy for endothelial glycocalyx observation using Alcian blue with silver enhancement.
Med Mol Morphol. 54(2):95-107, 2020 Open Access
DOI: 10.1007/s00795-020-00267-1
ナノスーツ法を応用したパラフィン切片観察
Kawasaki, H., Itoh, T., Takaku, Y., et al. The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy.
Lab Invest. 100(1): 161-173, 2020 Open Access
DOI: 10.1038/s41374-019-0309-7
デスクトップ型低真空走査電子顕微鏡の利点・特性を活かした
厚切り光顕用パラフィン切片：高解像立体構築解析法の開発
Informative three-dimensional survey of cell/tissue architectures in thick paraffin sections
by simple low-vacuum scanning electron microscopy
厚さ20〜30 µmに薄切した光顕用パラフィン切片に帯電防止処理を加えず、スライドガラスごと観察できるデスクトップ型低真空走査電子顕微鏡の利点・特性を活かし、生体を構成する組織や細胞を立体的に捉える新たな解析手法を開発しました。
厚切り光顕用パラフィン切片：高解像立体構築解析法の開発
Sawaguchi, A., Kamimura, T., Yamashita, A., Takahashi, N., Ichikawa, K., Aoyama, F., Asada, Y.: Informative three-dimensional survey of cell/tissue architectures in thick paraffin sections by simple low-vacuum scanning electron microscopy.
Sci Rep, 8(1): 7479, 2018 Open Access
DOI: 10.1038/s41598-018-25840-8
パパニコロー染色病理診断への応用
Yano, T., Soejima, Y., Sawabe, M. Application of low vacuum scanning electron microscopy for Papanicolaou-stained slides for cytopathology examinations.
Microscopy. 65: 269–273, 2016
https://doi.org/10.1093/jmicro/dfw005
IgA腎症における糸球体基底膜傷害の病理組織所見
Masuda, Y., Yamanaka, N., Ishikawa, A., et al. Glomerular basement membrane injuries in IgA nephropathy evaluated by double immunostaining for α5(IV) and α2(IV) chains of type IV collagen and low-vacuum scanning electron microscopy.
Clin Exp Nephrol. 19(3): 427-435, 2015
DOI: 10.1007/s10157-014-1008-8
小児ネフローゼ症候群病理組織診断への応用
Okada, S., Inaga, S., Kawabata, Y., et al. A novel approach to the histological diagnosis of pediatric nephrotic syndrome by low vacuum scanning electron microscopy.
Biomed Res. 35: 227–236, 2014 Free Article
https://doi.org/10.2220/biomedres.35.227
アルポート症候群病理組織診断への応用
Okada, S., Inaga, S., Kitamoto, K., et al. Morphological diagnosis of Alport syndrome and thin basement membrane nephropathy by low vacuum scanning electron microscopy.
Biomed Res. 35(5): 345-350, 2014 Free Article
DOI: 10.2220/biomedres.35.345
腎生検病理組織診断への応用
Miyazaki, H., Uozaki, H., Tojo, A., et al. Application of low-vacuum scanning electron microscopy for renal biopsy specimens.
Pathol Res Pract. 208(9): 503-509, 2012
DOI: 10.1016/j.prp.2012.05.006
白金ブルー染色法によるパラフィン切片観察
Inaga, S., Hirashima, S., Tanaka, K., et al. Low vacuum scanning electron microscopy for paraffin sections utilizing the differential stainability of cells and tissues with platinum blue.
Arch Histol Cytol. 72: 101–106, 2009 Free Article
https://doi.org/10.1679/aohc.72.101