English

Dermatology(reserch)

Information for Exchange Students

    

 

Name of the department: Department of Dermatology, Faculty of Medicine, University of Miyazaki

 

Name of the research project:

1.Molecular analysis of human T-cell lymphotropic virus type 1 (HTLV-1) proviarl load in cutaneous type Adult T-cell leukemia/lymphoma (ATLL).

 

2.Clinical presentations, prognostic factors, mortality and gene analysis in patients with Aeromonas spp. induced cellulitis in University of Miyazaki hospital.

 

3.Quantitative analysis of propionibacterial DNA in skin involvement from patients with sarcoidosis.

 

Head of the department: Professor Mitsuru Setoyama, MD, PhD

Tutor(s): Associate Professor Masahiro Amano, MD, PhD

Assistant Professor Sunao Tateyama, MD

Graduate Student Junko Nishimoto, MD

 

Language(s): English/Japanese

Duration of the project: two weeks or one month

Period of year: always

Acceptable number of students (per period/per year): 1-2/period, 2-4/year

 

Description of the project (including, relation to the main work of the laboratory, procedures and methods used in the project):

1.Adult T-cell leukemia/lymphoma (ATLL) is a malignant lymphoproliferative disorder caused by human T-cell lymphotropic virus type 1 (HTLV-1).  University of Miyazaki is located in an area of south-west Japan where HTLV-1 is endemic.  T lymphocytes, especially CD4+ T cells, are the main target of HTLV-1 in vivo and carry the majority of the HTLV-1 proviral DNA load.  HTLV-1 proviral DNA loads are measured by real-time polymerase chain reaction (PCR).  ATLL commonly involves the skin to vary 43% to 72% of patients.  We have investigated HTLV-I proviral DNA loads in the peripheral blood mononuclear cells and have analyzed the associations between HTLV-I proviral DNA loads and the clinical characteristics, especially skin involvement, among the ATLL patients.

 

2. Bacteria of the genus Aeromonas are ubiquitously found in aquatic environments and cause a variety of fish and human diseases. In humans, although gastroenteritis is the most frequent disease of Aeromonas infection, however, no dominant phylogenetic group of strains causing the gastroenteritis has yet been found. Strains belong to Aeromonas species also cause severe invasive (extraintestinal) diseases, including abscess, sepsis, necrotizing soft tissue infection, peritonitis, pneumonia, and life-threatening fulminant infections.

 We collected a total of 138 Aeromonas strains that were isolated in various regions of Japan and some other countries, from various sources, patients with intestinal or extraintestinal diseases and environment samples.

 Phylogenetic and virulence-related phenotypic analysis of 138 Aeromonas strains using the highly efficient and cost-effective, ‘next-generation’, sequencing technologies ( Roche (454) GS FLX sequence and Illumina genome analyzer ) and biochemical method and the evolutionary analysis of Aeromonas through an ecological and evlutionary view are performing in our laboratory.

 

3. Propionibacteriumacnes is thought to be a potential triggering agent of sarcodosis.

We have investigated Sarcoidosis-like reaction relations with PropionibacteriumAcnes .

 

Aim of the project:

1.The student(s) who joins our laboratory would work on the project proceeding at the term and learn techniques including handling and analysis of DNA by real time PCR.

 

2. Phylogenetic and virulence-related phenotypic analysis of 138 Aeromonas strains and the evolutionary analysis of Aeromonas through an ecological and evlutionary view

 

Requirements (if special knowledge is needed): No special knowledge is needed, but students who are interested in the research field are highly recommended.

 

Further information from:

Masahiro Amano