Information for Exchange Students
Research project: Biological and Molecular analysis of the Patients
with Congenital Immunodeficiency.
Head of the department: Professor Hiroyuki Nunoi, MD, PhD
Assistant Professor Hiroshi Moritake, PhD
Duration of the project: one month
Acceptable number of students: 1/period, 4/year
Description of the project:
Children are susceptible to bacterial, fungal and viral infections due to immaturity of immunity. More than 150 genes had been revealed to be responsible to congenital immunodeficiency. Among the diseases, we are very interested in an innate immunity, especially one of the neutrophile dysfunctions, namely Chronic Granulomatous Disease (CGD) that is caused by the defect of phagocyte superoxide production.
The student(s) who joins our laboratory would work on the project and learn techniques including biological functional assay and molecular biology as follows.
1. Isolation of neutrophile from your own blood.
2. FACS analysis of leukocytes subpopulation.
3. Analysis of neutrophile functions “chemotaxis, superoxide production, and bacterial killing.
4. Cloning, sequencing, PCR, and protein analysis of the genes, such as gp91phox, p22phox, p46phox, p67phox and p40phox which are responsible for CGD.
5. Establishment of the patient’s lymphocyte for your further studies using EB virus.
Aim of the project:
Innate immunity is very important for child immunity but still remain elucidated, especially, interaction of innate and acquired immunity. We have experienced many children with immunodeficiency. Along with analysis of the patients, you will learn neutrophile functions, molecular biology and DNA handling. DNA technology is on of the most favorite disciplines for many students. I hope that this project should become glimpse in to these biochemical bases.
Requirement: No special knowledge is needed, but students who are interested in the research field a highly recommended.