Applied Physiology

Information for Exchange Students


Name of the Department: Department of Applied Physiology, Faculty of Medicine, University of Miyazaki


Name of the research project: MicroRNAs in impaired healing of diabetic wounds- analysis and regulation


Head of the department: Professor Masugi Maruyama, MD, PhD


Tutors: Associate Professor Yuichi Nakajima, PhD

       Assistant Professor Harishkumar Madhyastha, PhD

       Assistant Professor Radha Madhyastha, PhD


Language(s): English/ Japanese

Duration of the project: One month

Period of year: All year except from Dec 25th to Jan 6th

Acceptable number of students (per period/ per year): 2/period; 4/year


Description of the project:

MicroRNAs (miRNAs) are small non-coding single stranded RNA molecules that function as negative regulators of genes at the translational level.  They play prominent roles in regulating diverse physiological processes including cell development, differentiation, migration, apoptosis, etc.  However, very little is known about the role of miRNAs in pathological conditions such as impaired healing of diabetic wounds.  Normal process of wound healing is disrupted in diabetic wounds.  Multiple factors contribute to the impaired healing of these wounds, the most important being less production of growth factors and retarded migration of cells towards the wound area. Since miRNAs are involved in processes such as angiogenesis, cell proliferation and migration, which are essential for proper healing of wounds, we study whether miRNAs contribute to impaired healing of diabetic wounds.  Using diabetic mouse models, we investigate the significance of miRNAs by studying their interaction with target genes and analyzing the molecular mechanisms underlying the regulations. 


The participant(s) will work on the project that is on-going at the time of joining the laboratory and gain hands-on experience on molecular biology techniques including:

(1)   miRNA isolation, PCR, DNA cloning and cellular transfection for functional analysis of the genes.

(2)   Electromobility shift assay (EMSA) for analysis of interaction between transcription factors and their target genes.

(3)   Analysis of miRNA regulation by natural (food) components as alternatives to chemical drugs.


Aim of the project:

              Molecular biology techniques form the basis for biomedical research. This project aims to provide hands-on experience to young research aspirants.


Requirements (if special knowledge is needed): Students with aptitude for research are preferred.


Further information from:

Professor Masugi Maruyama