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現職: |
宮崎大学医学部 学部講師 解剖学講座 組織細胞化学分野 |
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学位・資格:
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博士（工学）・甲種危険物取扱者，第一種衛生管理者 |
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略歴:
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2006年3月 東京工業大学生命理工学部 卒業 2008年3月 東京大学大学院工学系研究科バイオエンジニアリング専攻修士課程 修了 2008年9月 パルマ大学（イタリア）有機化学研究室 GCOE客員研究員 2009年4月 日本学術振興会特別研究員(DC2), 東京大学 2011年3月 東京大学大学院工学系研究科化学生命工学専攻博士課程 修了 2011年4月 日本学術振興会特別研究員(PD), 理化学研究所 横浜研究所 2013年1月 宮崎大学医学部 機能制御学講座 物質科学分野 特任助教 2016年4月 宮崎大学医学部 機能制御学講座 物質科学分野 助教 2021年4月 宮崎大学医学部 解剖学講座 組織細胞化学分野 助教 2022年4月 宮崎大学医学部 解剖学講座 組織細胞化学分野 学部講師 |
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専門分野:
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核酸化学 |
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加入学会:
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日本化学会，日本核酸化学会, 日本光医学・光生物学会, 日本組織細胞化学会, 日本解剖学会 |
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研究テーマ:
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主要論文:
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Ikenoue M, Choijookhuu N, Yano K, Fidya, Takahashi N, Ishizuka T, Shirouzu S, Yamaguma Y, Kai K, Higuchi K, Sawaguchi A, Nanashima A, Hishikawa Y. The crucial role of SETDB1 in structural and functional transformation of epithelial cells during regeneration after intestinal ischemia reperfusion injury. Histochem Cell Biol. 2024 Jan 13. doi: 10.1007/s00418-023-02263-9.
Fidya, Choijookhuu N, Ikenoue M, Yano K, Yamaguma Y, Shirouzu S, Kai K, Ishizuka T, Hishikawa Y. Protective role of estrogen through G-protein coupled receptor 30 in a colitis mouse model. Histochem Cell Biol. 2023 Oct 12. doi: 10.1007/s00418-023-02235-z. Shirouzu S, Sugita N, Choijookhuu N, Yamaguma Y, Takeguchi K, Ishizuka T, Tanaka M, Fidya, Kai K, Chosa E, Yamashita Y, Koshimoto C and Hishikawa Y. Pivotal role of High-Mobility Group Box 2 in ovarian folliculogenesis and fertility. J. Ovarian Res. 2022, 15, 133. doi: 10.1186/s13048-022-01071-4. He Z, Ishizuka T, Hishikawa Y, Xu Y. Click chemistry for fluorescence imaging via combination of a BODIPY-based ‘turn-on’ probe and a norbornene glucosamine. Chem. Commun. 2022, 58, 12479-12482. doi:10.1039/D2CC05359D. Choijookhuu N, Shibata Y, Ishizuka T, Xu Y, Koji T, Hishikawa Y. An advanced detection system for in situ hybridization using a fluorescence resonance energy transfer–based molecular beacon probe. Acta Histochem. Cytochem. 2022, 55, 119-128. doi: 10.1267/ahc.22-00075. Yamaguma Y, Sugita N, Choijookhuu N, Yano K, Lee D, Ikenoue M, Fidya, Shirouzu S, Ishizuka T, Tanaka M, Yamashita Y, Chosa E, Taniguchi N, Hishikawa Y. Crucial role of high-mobility group box 2 in mouse ovarian follicular development through estrogen receptor beta. Histochem. Cell Biol. 2022, 157, 359-369. doi: 10.1007/s00418-022-02074-4. Yum J H, Ishizuka T, Fukumoto K, Hori D, Bao H L, Xu Y, Sugiyama H, Park S: Systematic Approach to DNA Aptamer Design Using Amino Acid-Nucleic Acid Hybrids (ANHs) Targeting Thrombin. ACS Biomater. Sci. Eng. 2021, 7, 1338-1343. doi:10.1021/acsbiomaterials.1c00060. Bao H L, Ishizuka T, Yamashita A, Furukoji E, Asada Y, Xu Y: Improving thermodynamic stability and anticoagulant activity of a thrombin binding aptamer by incorporation of 8-trifluoromethyl-2′-deoxyguanosine. J. Med. Chem. 2021, 64, 711-718. doi:10.1021/acs.jmedchem.0c01711. Sasaki S, Ma Y, Ishizuka T, Bao H-L, Hirokawa T, Xu Y, Tera M, Nagasawa K: Linear consecutive hexaoxazoles as G4 ligands inducing chair-type anti-parallel topology of a telomeric G-quadruplex. RSC Advances 2020, 10, 43319-43323. doi:10.1039/d0ra09413g. Hayasaka K, Shibata T, Sugahara A, Momotake A, Matsui T, Neya S, Ishizuka T, Xu Y, Yamamoto Y: Characterization of structure and catalytic activity of a complex between heme and an all parallel-stranded tetrameric G-quadruplex formed from DNA/RNA chimera sequence d(TTA)r(GGG)dT. Bull. Chem. Soc. Jpn. 2020, 93, 621-629. doi:10.1246/bcsj.20200013. Zhang T, Yin C, Boyd D F, Quarato G, Ingram J P, Shubina M, Ragan K B, Ishizuka T, Crawford J C, Tummers B, Rodriguez D A, Xue J, Peri S, Kaiser W J, López C B, Xu Y, Upton J W, Thomas P G, Green D R, Balachandran S: Influenza virus Z-RNAs induce ZBP1-mediated necroptosis. Cell 2020, 180, 1115-1129.e1113. doi:10.1016/j.cell.2020.02.050. Liu H S, Ishizuka T, Kawaguchi M, Nishii R, Kataoka H, Xu Y: A nucleoside derivative 5-vinyluridine (VrU) for imaging RNA in cells and animals. Bioconjug. Chem. 2019, 30, 2958-2966. doi:10.1021/acs.bioconjchem.9b00643. Ishizuka T, Bao H L, Xu Y: 19F NMR spectroscopy for the analysis of DNA G-quadruplex structures using 19F-labeled nucleobase. Methods Mol. Biol. 2019, 2035, 407-433. doi:10.1007/978-1-4939-9666-7_26. Balasubramaniyam T, Ishizuka T, Xu Y: Stability and properties of Z-DNA containing artificial nucleobase 2′-O-methyl-8-methyl guanosine. Bioorg. Med. Chem. 2019, 27, 364-369. doi:10.1016/j.bmc.2018.12.005. Balasubramaniyam T, Ishizuka T, Xiao C D, Bao H L, Xu Y: 2’-O-methyl-8-methylguanosine as a Z-form RNA stabilizer for structural and functional study of Z-RNA. Molecules 2018, 23, 10.3390/molecules23102572. Ishizuka T, Yamashita A, Asada Y, Xu Y: Studying DNA G-quadruplex aptamer by 19F NMR. ACS Omega 2017, 2, 8843-8848. doi:10.1021/acsomega.7b01405. Ishizuka T, Zhao P Y, Bao H L, Xu Y: A multi-functional guanine derivative for studying the DNA G-quadruplex structure. Analyst 2017, 142, 4083-4088. doi:10.1039/c7an00941k. Xiao C D, Ishizuka T, Xu Y: Antiparallel RNA G-quadruplex formed by human telomere RNA containing 8-bromoguanosine. Sci. Rep. 2017, 7, 6695. doi:10.1038/s41598-017-07050-w. Liu X, Ishizuka T, Bao H L, Wada K, Takeda Y, Iida K, Nagasawa K, Yang D, Xu Y: Structure-dependent binding of hnRNPA1 to telomere RNA. J. Am. Chem. Soc. 2017, 139, 7533-7539. doi:10.1021/jacs.7b01599. Bao H L, Ishizuka T, Iwanami A, Oyoshi T, Xu Y: A simple and sensitive 19F NMR approach for studying the interaction of RNA G‐quadruplex with ligand molecule and protein. ChemistrySelect 2017, 2, 4170-4175. doi:10.1002/slct.201700711. Xiao C D, Ishizuka T, Zhu X Q, Li Y, Sugiyama H, Xu Y: Unusual topological RNA architecture with an eight-stranded helical fragment containing A-, G-, and U-tetrads. J. Am. Chem. Soc. 2017, 139, 2565-2568. doi:10.1021/jacs.6b12274. Bao H L, Ishizuka T, Sakamoto T, Fujimoto K, Uechi T, Kenmochi N, Xu Y: Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy. Nucleic Acids Res. 2017, 45, 5501-5511. doi:10.1093/nar/gkx109. Ishizuka T, Xu Y: Click chemistry takes a snapshot of DNA-RNA hybrid G-quadruplex in living cells. J. Nat. Sci. 2016, 2, e237. Ishizuka T, Liu H S, Ito K, Xu Y: Fluorescence imaging of chromosomal DNA using click chemistry. Sci. Rep. 2016, 6, 33217. doi:10.1038/srep33217. Ishizuka T, Xu Y, Komiyama M: Clipping of telomere from human chromosomes using a chemistry-based artificial restriction DNA cutter. Curr. Protoc. Nucleic Acid Chem. 2015, 61, 6.13.11-16.13.13. doi:10.1002/0471142700.nc0613s61. Xu Y, Suzuki Y, Ishizuka T, Xiao C D, Liu X, Hayashi T, Komiyama M: Finding a human telomere DNA-RNA hybrid G-quadruplex formed by human telomeric 6-mer RNA and 16-mer DNA using click chemistry: a protective structure for telomere end. Bioorg. Med. Chem. 2014, 22, 4419-4421. doi:10.1016/j.bmc.2014.05.053. Kameshima W, Ishizuka T, Minoshima M, Yamamoto M, Sugiyama H, Xu Y, Komiyama M: Conjugation of peptide nucleic acid with a pyrrole/imidazole polyamide to specifically recognize and cleave DNA. Angew. Chem. Int. Ed. 2013, 52, 13681-13684. doi:10.1002/anie.201305489. Ishizuka T, Xu Y, Komiyama M: A chemistry-based method to detect individual telomere length at a single chromosome terminus. J. Am. Chem. Soc. 2013, 135, 14-17. doi:10.1021/ja308481c. Xu Y, Ishizuka T, Yang J, Ito K, Katada H, Komiyama M, Hayashi T: Oligonucleotide models of telomeric DNA and RNA form a hybrid G-quadruplex structure as a potential component of telomeres. J. Biol. Chem. 2012, 287, 41787-41796. doi:10.1074/jbc.M112.342030. Ishizuka T, Kimoto M, Sato A, Hirao I: Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry. Chem. Commun. 2012, 48, 10835-10837. doi:10.1039/c2cc36293g. Ishizuka T, Yang J, Komiyama M, Xu Y: G-rich sequence-specific recognition and scission of human genome by PNA/DNA hybrid G-quadruplex formation. Angew. Chem. Int. Ed. 2012, 51, 7198-7202. doi:10.1002/anie.201201176. Xu Y, Ishizuka T, Kimura T, Komiyama M: A U-tetrad stabilizes human telomeric RNA G-quadruplex structure. J. Am. Chem. Soc. 2010, 132, 7231-7233. doi:10.1021/ja909708a. Ishizuka T, Tedeschi T, Corradini R, Komiyama M, Sforza S, Marchelli R: SSB-assisted duplex invasion of preorganized PNA into double-stranded DNA. ChemBioChem 2009, 10, 2607-2612. doi:10.1002/cbic.200900381. Xu Y, Ishizuka T, Kurabayashi K, Komiyama M: Consecutive formation of G-quadruplexes in human telomeric-overhang DNA: a protective capping structure for telomere ends. Angew. Chem. Int. Ed. 2009, 48, 7833-7836. doi:10.1002/anie.200903858. Miyajima Y, Ishizuka T, Yamamoto Y, Sumaoka J, Komiyama M: Origin of high fidelity in target-sequence recognition by PNA-Ce(IV)/EDTA combinations as site-selective DNA cutters. J. Am. Chem. Soc. 2009, 131, 2657-2662. doi:10.1021/ja808290e. Ishizuka T, Sforza S, Komiyama M: Promotion of strand invasion by utilizing entropically-favored PNA. Nucleic Acids Symp. Ser. 2009, 161-162. doi:10.1093/nass/nrp081. Ishizuka T, Otani K, Sumaoka J, Komiyama M: Strand invasion of conventional PNA to arbitrary sequence in DNA assisted by single-stranded DNA binding protein. Chem. Commun. 2009, 1225-1227. doi:10.1039/b813975j. Komiyama M, Aiba Y, Ishizuka T, Sumaoka J: Solid-phase synthesis of pseudo-complementary peptide nucleic acids. Nat. Protoc. 2008, 3, 646-654. doi:10.1038/nprot.2008.6. Ishizuka T, Komiyama M: Single-strand binding protein enhances invasion of a PNA strand to double-stranded DNA. Nucleic Acids Symp. Ser. 2008, 141-142. doi:10.1093/nass/nrn072. Miyajima Y, Ishizuka T, Komiyama M: Mismatch recognition in PNA double-duplex invasion. Nucleic Acids Symp. Ser. 2008, 125-126. doi:10.1093/nass/nrn064. Ishizuka T, Yoshida J, Yamamoto Y, Sumaoka J, Tedeschi T, Corradini R, Sforza S, Komiyama M: Chiral introduction of positive charges to PNA for double-duplex invasion to versatile sequences. Nucleic Acids Res. 2008, 36, 1464-1471. doi:10.1093/nar/gkm1154. Okada S, Arayama K, Murayama R, Ishizuka T, Hara K, Hirone N, Hata T, Urabe H: Iron-catalyst-switched selective conjugate addition of grignard reagents: α,β,γ,δ-unsaturated amides as versatile templates for asymmetric three-component coupling processes. Angew. Chem. Int. Ed. 2008, 47, 6860-6864. doi:10.1002/anie.200801928. |
