{"id":218,"date":"2014-11-16T16:38:07","date_gmt":"2014-11-16T07:38:07","guid":{"rendered":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english-proto\/?p=218"},"modified":"2026-01-29T11:44:37","modified_gmt":"2026-01-29T02:44:37","slug":"pharmacology","status":"publish","type":"post","link":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/laboratories\/pharmacology\/","title":{"rendered":"Pharmacology"},"content":{"rendered":"

Summary<\/h3>\n

The actin cytoskeleton is vital for a number of biological processes, including morphogenesis, motility, polarity establishment, and modulation of mechanical stress.\u00a0 These various tasks are performed by precise spatio-temporal control of actin polymerization\/depolymerization dynamics.\u00a0 However, little is known about the regulatory mechanisms of actin assembly in these processes in vivo.\u00a0 We use a multi-disciplinary approach that combines genetics, biochemistry, histology, and pharmacology to study these problems from the molecular to organism level.<\/p>\n

\"\"\"\"<\/p>\n

Research Projects<\/h3>\n
    \n
  1. Actin assembly mechanisms<\/li>\n
  2. Actin dynamics in cardiomyocytes and non-muscle cells<\/li>\n<\/ol>\n

    Lab Techniques<\/h3>\n

    \"\"<\/p>\n

      \n
    1. Basic molecular biology techniques<\/li>\n
    2. Basic techniques for mammalian cell culture<\/li>\n
    3. Maintenance and handling of genetically modified mice<\/li>\n
    4. Expression and purification of recombinant proteins<\/li>\n
    5. Primary culture of cardiomyocytes and neurons<\/li>\n
    6. Morphological and histological observation techniques<\/li>\n<\/ol>\n

      Publications<\/h3>\n
        \n
      1. Syaban MFR,\u00a0et al<\/span>. Structural basis underlying the autoinhibition of the formin FHOD1 and its phosphorylation-dependent activation.\u00a0J. Biol. Chem.<\/em><\/strong>\u00a02026; 302: 111109.\u00a0doi: 10.1016\/j.jbc.2025.111109.<\/a><\/li>\n
      2. Nakagawa H,\u00a0et al<\/span>. The expression of the formin Fhod3 in mouse tongue striated muscle.\u00a0Cell Struct. Funct.,<\/em><\/strong>\u00a02024; 49: 111-122<\/span>.\u00a0doi: 10.1247\/csf.24044.<\/a><\/li>\n
      3. Sakata K,\u00a0et al<\/span>.\u00a0 Differential effects of the formin inhibitor SMIFH2 on contractility and Ca2+<\/sup>\u00a0handling in frog and mouse cardiomyocytes.\u00a0Genes Cells.<\/strong><\/em>\u00a02021; 26:583-595<\/span>.\u00a0doi: 10.1111\/gtc.12873.<\/a><\/li>\n
      4. Sulistomo HW,\u00a0et al<\/span>. Fhod3 controls the dendritic spine morphology of specific subpopulations of pyramidal neurons in the mouse cerebral cortex.\u00a0Cerebral Cortex<\/em><\/strong>\u00a02021;31:2205\u20132219.\u00a0doi: 10.1093\/cercor\/bhaa35<\/li>\n
      5. Sanematsu F,\u00a0<\/u>et al<\/span>.\u00a0Fhod1, an actin-organizing formin family protein, is dispensable for cardiac development and function in mice.\u00a0Cytoskeleton (Hoboken).\u00a0<\/strong><\/em>2019 Feb;76(2):219-229.\u00a0doi: 10.1002\/cm.21523.<\/a><\/li>\n
      6. Matsuyama S,\u00a0et al<\/span>. Interaction between cardiac myosin-binding protein C and formin Fhod3.\u00a0Proc Natl Acad Sci U S A.<\/strong><\/em>\u00a02018 ; 115: E4386-95.\u00a0doi: 10.1073\/pnas.1716498115.<\/a><\/li>\n
      7. Sulistomo HW,\u00a0et al<\/span>. Formin homology 2 domain-containing 3 controls neural plate morphogenesis in mouse cranial neurulation by regulating multidirectional apical constriction.\u00a0J Biol Chem.<\/strong><\/em>\u00a02019; 294: 2924-2934.<\/span>\u00a0<\/li>\n
      8. Ushijima T,\u00a0et al<\/span>. The actin-organizing formin protein Fhod3 is required for postnatal development and functional maintenance of the adult heart in mice.\u00a0J Biol Chem.<\/strong><\/em>\u00a02018;\u00a0<\/span>293:148-162.<\/li>\n
      9. \u200b<\/span>Kan-o M, et al.\u00a0 Mammalian formin Fhod3 plays an essential role in cardiogenesis by organizing myofibrillogenesis.\u00a0\u00a0Biol. Open<\/i><\/b><\/i><\/b>\u00a02012;\u00a0<\/span>1:<\/span>\u00a0889-896.<\/span><\/li>\n
      10. Taniguchi\u00a0K, et al.<\/span>,\u00a0<\/span>Mammalian Formin Fhod3 Regulates Actin Assembly and Sarcomere Organization in Striated Muscles.\u00a0<\/span>J Biol Chem.<\/strong><\/em>\u00a02009;\u00a0<\/span>284:<\/span>\u00a029873-29881.<\/span><\/li>\n
      11. Takeya R,<\/span>\u00a0et al.\u00a0The mammalian formin FHOD1 is activated through phosphorylation by ROCK and mediates thrombin-induced stress fibre formation in endothelial cells.\u00a0<\/span>EMBO J\u00a0<\/i><\/b>2008; 27:<\/span>\u00a0618-628.<\/span><\/li>\n<\/ol>\n\n","protected":false},"excerpt":{"rendered":"

        Summary The actin cytoskeleton is vital for a number of biological processes, including morphogenesis, motilit […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false},"categories":[1],"tags":[],"_links":{"self":[{"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/posts\/218"}],"collection":[{"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/comments?post=218"}],"version-history":[{"count":21,"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/posts\/218\/revisions"}],"predecessor-version":[{"id":1469,"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/posts\/218\/revisions\/1469"}],"wp:attachment":[{"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/media?parent=218"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/categories?post=218"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english\/wp-json\/wp\/v2\/tags?post=218"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}