Dendritic cells (DCs) are leukocytes that consist of heterogeneous subsets, including conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Accumulating evidence suggests that DCs are essential antigen-presenting cells (APCs) that link innate and adaptive immunity under inflammatory conditions and also induce immunological tolerance to maintain immune homeostasis under steady-state conditions. Furthermore, DC functions can be controlled by various intrinsic factors and extrinsic stimulations. However, the precise functional role of each DC subset in immune responses remains unclear. Our goal is to clarify the role of DC subsets in the immune system and to identify the molecular basis for the regulation of their function.
- Analysis of the role of DC subsets in the control of immune response and pathogenesis.
- Analysis of the molecular basis for the regulation of the function of DC subsets.
- Development of a novel cell-based immunotherapy for the immunopathogenic diseases (inflammatory and autoimmune diseases, allergy, graft rejection, and GVHD).
- Development of a novel tumor vaccine formulation.
- Isolation and culture of immune cells from organ and tissue.
- Assay for immune cell function in vivo and in vitro.
- Detection of the expression of gene and protein (e.g., PCR, Southern blotting, Western blotting, ELISA).
- Generation of gene-modified protein, cells and mouse by molecular biology techs (e.g., mutagenesis, retroviral gene transduction, gene knock-out / knock-in) .
- Flow cytometry and cell sorting (immunolabeling [FACS and MACS]).
- Histological observation (e.g., immunostaining [confocal microscopy]).
- Assay for immunopathogenesis in murine models.
- Centre d’Immunologie de Marseille-Luminy, UM2 Aix-Marseille Université, Marseille, France.
- Department of Immunology and Microbial Science, The Scripps Research Institute, USA.
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