{"id":491,"date":"2026-09-28T09:35:59","date_gmt":"2026-09-28T00:35:59","guid":{"rendered":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english-demo\/?page_id=491"},"modified":"2026-09-28T11:02:27","modified_gmt":"2026-09-28T02:02:27","slug":"microbial-biochemistry-fsrc","status":"publish","type":"page","link":"http:\/\/www.med.miyazaki-u.ac.jp\/home\/english-demo\/departments\/laboratories\/microbial-biochemistry-fsrc\/","title":{"rendered":"Microbial Biochemistry, FSRC"},"content":{"rendered":"\n

Frontier Science Research Center<\/strong>
Microbial Biochemistry
https:\/\/sites.google.com\/view\/shiolab-univ-miyazaki\/home<\/a><\/p>\n\n\n\n

Summary<\/h2>\n\n\n\n

Rising the antibiotic-resistance bacteria such as \u201csuper-bugs\u201d is one of the most serious global issues. Gram-negative bacteria have beta-barrel membrane protein in the outer membrane that are an important role for post-pathogen interaction and virulence functions as well as cell viability. Our interests are especially focused on how beta-barrel membrane proteins are assembled into the outer membranes of bacteria. In order to address this question, in our lab uses the novel biochemical method termed \u201cEMM assembly assay\u201d and other biochemical approaches. Aim of our lab is developing the new drugs targeting beta-barrel membrane protein assembly.<\/p>\n\n\n\n

Research projects<\/h2>\n\n\n\n
  1. Functional and Structural Characterization of the Escherichia coli Beta-Barrel Assembly Machinery (BAM) Complex<\/li>
  2. Identification of Outer Membrane Factors Essential for In Vivo Growth of Gram-Negative Bacteria<\/li>
  3. Culture-Independent Structural and Functional Analysis of Outer Membrane Proteins from Unculturable Gram-Negative Bacteria<\/li><\/ol>\n\n\n\n

    Lab Techniques<\/h2>\n\n\n\n

    Bacterial Genetics, PCR, Protein Purification, in vitro reconstitution assay, chemical screening<\/p>\n\n\n\n

    Collaborators<\/h3>\n\n\n\n

    Trevor Lithgow lab (Monash Uni, Australia)
    Hsin Hui Shen lab (Monash Uni, Australia)
    Rhys Grinter lab (Melbourne Uni, Australia)
    Lorenzo Giacani lab (Wahington Uni, USA)<\/p>\n\n\n\n

    Publications<\/h2>\n\n\n\n
    1. Miyazaki R, Kohga H, Matsuoka N, Maruno Y, Yoshimoto W, Takahashi YS, Yanto DHY, Nugraha Y, Shigematsu H, Shiota T, Tsukazaki T. Cryo-EM structures of the SurA\u2013BAM complex reveal conformational changes in outer membrane protein assembly. Nature Communications. 2026 Sep 4;17(1):9061. PMID: 42697891, DOI: 10.1038\/s41467-026-76843-3<\/li>
    2. Germany E, Shiota T. Analysis of protein-protein interaction of the mitochondrial translocase at work by using technically effective BPA photo-crosslinking method. Methods in Enzymology. 2024;707:237-256. PMID: 39488377, DOI: 10.1016\/bs.mie.2024.07.062<\/li>
    3. Maruno Y, Germany EM, Nakajima Y, Ohyama R, Masumura Y, Shiota T. The N-terminal extension region of BamE coordinates interaction between the beta-barrel domain of BamA and BamD for efficient assembly. bioRxiv [Preprint]. 2024 Oct 24. DOI: 10.1101\/2024.10.23.619864<\/li>
    4. Aoki E, Germany E, Shiota T. In Vitro Reconstruction of Bacterial \u03b2-Barrel Membrane Protein Assembly Using E. coli Microsomal (Mid-Density) Membrane. Methods in Molecular Biology. 2024;2778:65-81. PMID: 38478272, DOI: 10.1007\/978-1-0716-3734-0_5<\/li>
    5. Germany EM, Thewasano N, Imai K, Maruno Y, Bamert RS, Stubenrauch CJ, Dunstan RA, Ding Y, Nakajima Y, Lai XF, Webb CT, Hidaka K, Tan KS, Shen HH, Lithgow T, Shiota T. Dual recognition of multiple signals in bacterial outer membrane proteins enhances assembly and maintains membrane integrity. eLife. 2024 Jan 12;12:RP90274. PMID: 38226797, DOI: 10.7554\/eLife.90274<\/li>
    6. Koyamatsu D, Otsubo M, Ohira T, Sato MP, Suzuki-Masuko H, Shiota T, Takano KT, Ozeki M, Otsuka K, Ogura Y, et al. Molecular characterization of SrSTP14, a sugar transporter from thermogenic skunk cabbage, and its possible role in developing pollen. Physiologia Plantarum. 2023 Jul-Aug;175(4):e13957. PMID: 37338180, DOI: 10.1111\/ppl.13957<\/li>
    7. Thewasano N, Germany EM, Maruno Y, Nakajima Y, Shiota T. Categorization of Escherichia coli outer membrane proteins by dependence on accessory proteins of the Beta-barrel assembly machinery complex. Journal of Biological Chemistry (JBC). 2023 Jul;299(7):104880. PMID: 37196764, DOI: 10.1016\/j.jbc.2023.104821<\/li>
    8. Ikegawa Y, Combet C, Groussin M, Navratil V, Safar-Remali S, Shiota T, Aouacheria A, Yoo SK. Evidence for existence of an apoptosis-inducing BH3-only protein, sayonara, in Drosophila. The EMBO Journal. 2023 Apr 17;42(8):e110454. PMID: 36727601, DOI: 10.15252\/embj.2021110454<\/li>
    9. Araiso Y, Tsutsumi A, Qiu J, Imai K, Shiota T, Song J, Lindau C, Wenz LS, Sakaue H, Yunoki K, Kawano S, Becker T, Pfanner N, Wiedemann N, Kohda D, Endo T. Structure of the mitochondrial TOM complex. Nature. 2019 Mar;567(7747):255-260. PMID: 30842658, DOI: 10.1038\/s41586-019-0980-0<\/li>
    10. Sakaue H, Shiota T, Ishizaka M, Kawano S, Tamura Y, Endo T. Visualizing the Rapid-Response Machinery Inserting Transmembrane Peptides into the Mitochondrial Outer Membrane. Molecular Cell. 2019 Feb 21;73(4):813-824.e5. PMID: 30661980, DOI: 10.1016\/j.molcel.2018.12.016<\/li>
    11. Shiota T, Imai K, Qiu J, Hewitt VL, Tan K, Shen HH, Sakiyama N, Fukuda Y, Nishikawa S, Yamamoto H, Lithgow T, Endo T. Molecular architecture of the active mitochondrial protein translocase complex. Science. 2015 Sep 25;349(6255):1538-1542. PMID: 26338799, DOI: 10.1126\/science.aac6428<\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"

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