Frontier Science Research Center,  Electron Microscope Lab

University of Miyazaki

研究業績

【英文原著・総説】

Nishi, M., Aoyama, F., Kisa, F., Zhu, H., Sun, M., Lin, P., Ohta, H., Van, B., Yamamoto, S., Kakizawa, S., Sakai, H., Ma, J., Sawaguchi, A., Takeshima, H.: TRIM50 regulates vesicular trafficking for acid secretion in gastric parietal cells.

J Bioll Chem, 28; 287(40): 33523-32, 2012

Kuroki, M., Nakada, H., Yamashita, A., Sawaguchi, A., Uchino, N., Sato, S., Asanuma, T., Asada, Y., Tamura, S.: Loss of cellular viability in areas of ground-glass opacity on computed tomography images immediately after pulmonary radiofrequency ablation in rabbits.

Jap J Radiol, 30: 323-330, 2012

Ohta, K., Higashi, R., Sawaguchi, A., Nakamura, KI.: Helical arrangement of filaments in microvillar actin bundles.

J Struc Biol, 177: 513-519, 2012

Kawaguchi, M., Sawaguchi, A., Kataoka, H.: Membrane-bound serine protease inhibitor HAI-1 is required for maintenance of intestinal epithelial integrity.

Am J Pathol, 179: 1815-1826, 2011

Aoyama, F., Sawaguchi, A.: Functional transformation of gastric parietal cells and intracellular trafficking of ion channels/transporters in the apical canalicular membrane associated with acid secretion.

Biol Pharm Bull 34: 813-816, 2011

Takayama, N., Nishimura, S., Nakamura, S., Shimizu, T., Ohnishi, R., Endo, H., Yamaguchi, T., Otsu, M., Nishimura, K., Nakanishi, M., Sawaguchi, A., Nagai, R., Takahashi, K., Yamanaka, S., Nakauchi, H., Eto, K.: Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells.

J Exp Med 207: 2817-2830, 2010

Sawaguchi, A.: Immunoelectron microscopy of cryofixed freeze substituted mammalian tissue culture cells.

Methods in Molecular Biology. 657: 181-190, 2010

Takizawa, H., Nishimura, S., Takayama, N., Oda, A., Nishikii, H., Morita, Y., Kakinuma, S., Yamazaki, S., Okamura, S., Tamura, N., Goto, S., Sawaguchi, A., Manabe, I., Takatsu, K., Nakauchi, H., Takaki, S., Eto, K.: Lnk/Sh2b3 regulates integrin αIIbβ3 outside-in signaling in platelets leading to stabilization of developing thrombus in vivo.

J Clin Invest 120: 179-190, 2010

Asadulghani, M., Ogura, Y., Ooka, T., Itoh, T., Sawaguchi, A., Iguchi, A., Nakayama, K., Hayashi, T.: The defective prophage pool of Escherichia coli O157: prophage-prophage interaction potentiate horizontal transfer of virulence determinants.

PLoS Pathog, 5: e1000408, 2009

Sawaguchi, A., Aoyama, F., Ide S., Suganuma, T.: Capsule-supporting ring: a new device for resin embedding of glass-mounted specimens.

J Microsc, 234: 113-117, 2009

Sawaguchi, A., Aoyama, F., Ide S., Goto, Y., Suganuma, T.: A new device for high-pressure freezing of cultured cell monolayer using 10 µm-thin stainless discs as both culture plate and specimen carrier.

J Electron Mircosc 57: 203-206, 2008

Nishikii, H., Eto, K., Tamura, N., Hattori, K., Heissig, B., Kanaji, T., Sawaguchi, A., Goto, S., Ware, J., Nakauchi, H.: Metalloproteinase regulation improves in vitro generation of efficacious platelets from mouse embryonic stem cells.

J Exp Med 205: 1917-1927, 2008

Takayama, N.,Nishikii, H., Usui, J., Tsukui, H., Sawaguchi, A., Hiroyama, T., Eto, K., Nakauchi, H.: Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors.

Blood 111: 5298-5306, 2008

Aoyama, F., Sawaguchi, A., Ide, S., Kitamura, K., Suganuma, T.: Exfoliation of gastric pit-parietal cells into the gastric lumen associated with a stimulation of isolated rat gastric mucosa in vitro: a morphological study by the application of cryotechniques.

Histochem Cell Biol 129: 785-793, 2008

Ohashi, M., Ide, S., Sawaguchi, A., Kimitsuki, T., Komune, S., Suganuma, T.: Histochemical localization of the extracellular matrix components in the annular ligament of rat stapediovestibular joint with special reference to fibrillin, 36-kD microfibril-associated glycoprotein (MAGP-36), and hyaluronic acid.

Med Mol Morphol 41: 28-33, 2008

Sawaguchi, A., Aoyama, F., Ohashi, M., Ide S., Suganuma, T.: Ultrastructural transformation of gastric parietal cells reverting from the active to the resting state of acid secretion revealed in isolated rat gastric mucosa model processed by high-pressure freezing.

J Electron Microsc 55: 97-105, 2006

Ohashi, M., Sawaguchi, A., Ide, S., Kimitsuki, T., Komune, S., Suganuma, T.: Histochemical characterization of the rat ossicular joint cartilage with a special reference to stapediovestibular joint.

Acta Histochem Cytochem 38: 387-392, 2005

Sawaguchi, A., Aoyama, F., Ide, S., Suganuma, T.: The cryofixation of isolated rat gastric mucosa provides new insights into the functional transformation of gastric parietal cells: an in vitro experimental model study.

Arch Histol Cytol 68: 151-160, 2005

Sawaguchi, A., Ide, S., Suganuma, T.: Application of 10-µm thin stainless foil to a new assembly of the specimen carrier in high pressure freezing.

J Electron Microsc, 54: 143-146, 2005

Sawaguchi, A., McDonald, K.L., Forte, J.G.: High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells.

J Histochem Cytochem, 52: 77-86, 2004

Sawaguchi, A., Yao, X., Forte, J.G., McDonald, K.L.: Direct attachment of cell suspensions to high pressure freezing specimen planchettes.

J Microsc 212: 13-20, 2003

Sawaguchi, A., McDonald, K.L., Karvar, S., Forte, J.G.: A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells.

J Microsc 208: 158-166, 2002

Yuchi, H., Suganuma, T., Sawaguchi, A., Ide, S., Kawano, J., Aoki, T., Kitamura, K., Eto, T.: Cryofixation processing is an excellent method to improve the retention of adrenomedullin antigenicity.

Histochem Cell Biol 118: 259-265, 2002

Sawaguchi, A., Ishihara, K., Kawano, J., Oinuma, T., Hotta, K., Suganuma, T.: Fluid dynamics of the excretory flow of zymogenic and mucin contents in rat gastric gland processed by high pressure freezing/freeze substitution.

J Histochem Cytochem 50: 223-234, 2002

Sawaguchi, A., Tojo, H., Kawano, J., Okamoto, M., Suganuma, T.: Immunocytochemical demonstration of the secretory dynamics of zymogenic contents in rat gastric gland processed by high-pressure freezing/freeze substitution, with special references to phospholipase A2 and phospholipase Cg1.

Histochem Cell Biol. 116: 361-369, 2001

Sawaguchi, A., Ide, S., Goto, Y., Kawano, J., Oinuma, T., Suganuma, T.: A simple contrast enhancement by potassium permanganate oxidation for Lowicryl K4M ultrathin sections prepared by high pressure freezing/freeze substitution.

J Microsc 201: 77-83, 2001

Date, Y., Kojima, M., Hosoda, H., Sawaguchi, A., Mondal, M.S., Suganuma, T., Matsukura, S., Kangawa, K., Nakazato, M.: Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans.

Endocrinology 141: 4255-4261, 2000

Sawaguchi, A., Ide, S., Kawano, J., Nagaike, R., Oinuma, T., Tojo, H., Okamoto, M., Suganuma, T.: Reappraisal of potassium permanganate oxidation applied to Lowicryl K4M embedded tissues processed by high pressure freezing / freeze substitution, with special reference to differential staining of the zymogen granules of rat gastric chief cells.

Arch. Histol. Cytol. 62: 447-458, 1999

Sawaguchi, A., Idate, Y., Ide, S., Kawano, J., Nagaike, R., Oinuma, T., Suganuma, T.: Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina.

J. Histochem. Cytochem. 47: 919-928, 1999

【和文総説】

澤口　朗、豊嶋　典世：高圧凍結技法による胃粘膜の超微形態研究

顕微鏡 45: 130-132, 2010

澤口　朗、菅沼龍夫：高圧凍結技法の新展開─培養細胞への応用

顕微鏡 403: 204-206, 2005

菅沼龍夫、澤口　朗：凍結技法における新展開─高圧凍結技法

細 胞 35: 32-35, 2003

菅沼龍夫、澤口　朗、津山新一郎：高圧凍結・凍結置換法による形態と組織化学

電子顕微鏡 36: 99-101, 2001

ラボ紹介　Introduction