研究室紹介 Introduction
研 究 Research
教 育 Education
研究業績
【英文原著・総説】
Nemoto, T., Toyoshima-Aoyama, F., Yanagita, T., Maruta, T., Fujita, H., Koshida, T., Yonaha, T., Wada, A., Sawaguchi, A., Murakami, M.: New insights concerning insulin synthesis and its secretion in rat hippocampus and cerebral cortex: Amyloid-β1-42-induced reduction of proinsulin level via glycogen synthase kinase-3β.
Cell Signal, 26(2): 253-259, 2014
Hirose, S., Takayama, N., Nakamura, S., Nagasawa, K., Ochi, K., Hirata, S., Yamazaki, S., Yamaguchi, T., Otsu, M., Sano, S., Takahashi, N., Sawaguchi, A., Ito, M., Kato, T., Nakauchi, H., Eto, K.: Immortalization of Erythroblasts by c-MYC and BCL-XL Enables Large-Scale Erythrocyte Production from Human Pluripotent Stem Cells.
Stem Cell Reports. 2013 Dec 5;1(6):499-508. doi: 10.1016/j.stemcr.2013.10.010.
Nemoto, T., Toyoshima-Aoyama, F., Ueda, Y., Ohba, T., Yanagita, T., Watanabe, H., Shirasaka, T., Tsuneyoshi, I., Ishida, Y., Hirota, K., Sawaguchi, A., Murakami, M.:Involvement of the orexin system in adrenal sympathetic regulation.
Pharmacology 91(5-6) : 250-258, 2013
Akieda-Asai, S., Sugiyama, M., Miyazawa, T., Koda, S., Okano, I., Senba, K., Poleni, P.E., Hizukuri, Y., Okamoto, A., Yamahara, K., Mutoh, E., Aoyama, F., Sawaguchi, A., Furuya, M., Miyazato, M., Kangawa, K., Date, Y.: Involvement of guanylin and GC-C in rat mesenteric macrophages in resistance to high-fat diet.
J Lipid Res, 54(1): 85-96, 2013
Nishi, M., Aoyama, F., Kisa, F., Zhu, H., Sun, M., Lin, P., Ohta, H., Van, B., Yamamoto, S., Kakizawa, S., Sakai, H., Ma, J., Sawaguchi, A., Takeshima, H.: TRIM50 regulates vesicular trafficking for acid secretion in gastric parietal cells.
J Bioll Chem, 287(40): 33523-32, 2012
Kuroki, M., Nakada, H., Yamashita, A., Sawaguchi, A., Uchino, N., Sato, S., Asanuma, T., Asada, Y., Tamura, S.: Loss of cellular viability in areas of ground-glass opacity on computed tomography images immediately after pulmonary radiofrequency ablation in rabbits.
Jap J Radiol, 30: 323-330, 2012
Tajiri, S., Fukui, T., Sawaguchi, A., Yoshinaga, K.: Cell- and region-specific expression of sugar chains in the mouse epididymal epithelium using lectin histochemistry combined with immunohistochemistry.
Okajimas Folia Anatomica Japonica, 88: 141-146, 2012
Tajiri, S., Fukui, T., Sawaguchi, A., Yoshinaga, K.: Changes in lectin-binding sites on epididymal cells during postnatal development of the muse.
Okajimas Folia Anatomica Japonica, 88: 153-157, 2012
Ohta, K., Higashi, R., Sawaguchi, A., Nakamura, KI.: Helical arrangement of filaments in microvillar actin bundles.
J Struc Biol, 177: 513-519, 2012
Kawaguchi, M., Sawaguchi, A., Kataoka, H.: Membrane-bound serine protease inhibitor HAI-1 is required for maintenance of intestinal epithelial integrity.
Am J Pathol, 179: 1815-1826, 2011
Aoyama, F., Sawaguchi, A.: Functional transformation of gastric parietal cells and intracellular trafficking of ion channels/transporters in the apical canalicular membrane associated with acid secretion.
Biol Pharm Bull 34: 813-816, 2011
Yoshida, A., Nagayasu, E., Nishimaki, A., Sawaguchi, A., Yanagawa, S., Maruyama, H.: Transcripts analysis of infective larvae of an intestinal nematode, Stongyloides venezuelensis.
Parasitol Int 60: 75-83, 2011
Takayama, N., Nishimura, S., Nakamura, S., Shimizu, T., Ohnishi, R., Endo, H., Yamaguchi, T., Otsu, M., Nishimura, K., Nakanishi, M., Sawaguchi, A., Nagai, R., Takahashi, K., Yamanaka, S., Nakauchi, H., Eto, K.: Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells.
J Exp Med 207: 2817-2830, 2010
Sawaguchi, A.: Immunoelectron microscopy of cryofixed freeze substituted mammalian tissue culture cells.
Methods in Molecular Biology. 657: 181-190, 2010
Takizawa, H., Nishimura, S., Takayama, N., Oda, A., Nishikii, H., Morita, Y., Kakinuma, S., Yamazaki, S., Okamura, S., Tamura, N., Goto, S., Sawaguchi, A., Manabe, I., Takatsu, K., Nakauchi, H., Takaki, S., Eto, K.: Lnk/Sh2b3 regulates integrin αIIbβ3 outside-in signaling in platelets leading to stabilization of developing thrombus in vivo.
J Clin Invest 120: 179-190, 2010
Asadulghani, M., Ogura, Y., Ooka, T., Itoh, T., Sawaguchi, A., Iguchi, A., Nakayama, K., Hayashi, T.: The defective prophage pool of Escherichia coli O157: prophage-prophage interaction potentiate horizontal transfer of virulence determinants.
PLoS Pathog, 5: e1000408, 2009
Sawaguchi, A., Aoyama, F., Ide S., Suganuma, T.: Capsule-supporting ring: a new device for resin embedding of glass-mounted specimens.
J Microsc, 234: 113-117, 2009
Umekita, K., Hidaka, T., Ueno, S., Takajo, I., Kai, Y., Nagatomo, Y., Sawaguchi, A., Suganuma, T., Okayama, A.: Leukocytapheresis (LCAP) decreases the level of platelet-derived microparticles (MPs) and increases the level of granulocytes-derived MPs: a possible connection with the effect of LCAP on rheumatoid arthritis.
Mod Rheumatol, 19(3) 265-272, 2009
Huang, L., Adachi, T., Shimizu, Y., Goto, Y., Toyama, J., Tanaka, H., Akashi, R., Sawaguchi, A., Iwata, H., Haga, T.: Characterization of lectin isolated from Momordica charantia seed as a B cell activator.
Immunol. Lett, 121:148-156, 2008
Sawaguchi, A., Aoyama, F., Ide S., Goto, Y., Suganuma, T.: A new device for high-pressure freezing of cultured cell monolayer using 10 µm-thin stainless discs as both culture plate and specimen carrier.
J Electron Mircosc 57: 203-206, 2008
Nagaike, K., Kawaguchi, M., Takeda, N., Fukushima, T., Sawaguchi, A., Hohama, K., Setoyama, M., Kataoka, H.: Defect of hepatocyte growth factor activator inhibitor type 1/serine protease inhibitor, Kunitz type 1 (Hai-1/Spint1) leads to ichthyosis-like condiction and abnormal hair development in mice.
Am J Pathol 173: 1464-1475, 2008
Nishikii, H., Eto, K., Tamura, N., Hattori, K., Heissig, B., Kanaji, T., Sawaguchi, A., Goto, S., Ware, J., Nakauchi, H.: Metalloproteinase regulation improves in vitro generation of efficacious platelets from mouse embryonic stem cells.
J Exp Med 205: 1917-1927, 2008
Takayama, N.,Nishikii, H., Usui, J., Tsukui, H., Sawaguchi, A., Hiroyama, T., Eto, K., Nakauchi, H.: Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors.
Blood 111: 5298-5306, 2008
Aoyama, F., Sawaguchi, A., Ide, S., Kitamura, K., Suganuma, T.: Exfoliation of gastric pit-parietal cells into the gastric lumen associated with a stimulation of isolated rat gastric mucosa in vitro: a morphological study by the application of cryotechniques.
Histochem Cell Biol 129: 785-793, 2008
Ohashi, M., Ide, S., Sawaguchi, A., Kimitsuki, T., Komune, S., Suganuma, T.: Histochemical localization of the extracellular matrix components in the annular ligament of rat stapediovestibular joint with special reference to fibrillin, 36-kD microfibril-associated glycoprotein (MAGP-36), and hyaluronic acid.
Med Mol Morphol 41: 28-33, 2008
Tsubokawa, D., Goso, Y., Sawaguchi, A., Kurihara, M., Ichikawa, T., Sato, M., Suganuma, T., Hotta, K., Ishihara, K.: An anti-mucin monoclonal antibody, PGM34, against 6-sulfated blood-group H type 2 antigen on the carbohydrate moiety of mucin: Analysis of the epitope sequence and immunohistochemical study.
FEBS Journal 274: 1833-1848, 2007
Sawaguchi, A., Aoyama, F., Ohashi, M., Ide S., Suganuma, T.: Ultrastructural transformation of gastric parietal cells reverting from the active to the resting state of acid secretion revealed in isolated rat gastric mucosa model processed by high-pressure freezing.
J Electron Microsc 55: 97-105, 2006
Ohashi, M., Sawaguchi, A., Ide, S., Kimitsuki, T., Komune, S., Suganuma, T.: Histochemical characterization of the rat ossicular joint cartilage with a special reference to stapediovestibular joint.
Acta Histochem Cytochem 38: 387-392, 2005
Sawaguchi, A., Aoyama, F., Ide, S., Suganuma, T.: The cryofixation of isolated rat gastric mucosa provides new insights into the functional transformation of gastric parietal cells: an in vitro experimental model study.
Arch Histol Cytol 68: 151-160, 2005
Inagaki-Ohara, K., Sawaguchi, A., Suganuma, T., Matsuzaki, G., Nawa, Y.: Intraepithelial lymphocytes express junctional molecules in murine small intestine.
Biochem and Biophys Res Comm, 331: 977-983, 2005
Sawaguchi, A., Ide, S., Suganuma, T.: Application of 10-µm thin stainless foil to a new assembly of the specimen carrier in high pressure freezing.
J Electron Microsc, 54: 143-146, 2005
Nagaike, R., Sawaguchi, A., Kawano, J., Aoyama, F., Oinuma, T., Suganuma, T.: Regeneration of gastric mucosa during ulcer healing follows pathways that correspond to the ontogenetic course of rat fundic glands.
Virchows Arch, 445: 580-588, 2004
Sawaguchi, A., McDonald, K.L., Forte, J.G.: High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells.
J Histochem Cytochem, 52: 77-86, 2004
Sawaguchi, A., Yao, X., Forte, J.G., McDonald, K.L.: Direct attachment of cell suspensions to high pressure freezing specimen planchettes.
J Microsc 212: 13-20, 2003
Sawaguchi, A., McDonald, K.L., Karvar, S., Forte, J.G.: A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells.
J Microsc 208: 158-166, 2002
Yuchi, H., Suganuma, T., Sawaguchi, A., Ide, S., Kawano, J., Aoki, T., Kitamura, K., Eto, T.: Cryofixation processing is an excellent method to improve the retention of adrenomedullin antigenicity.
Histochem Cell Biol 118: 259-265, 2002
Sawaguchi, A., Ishihara, K., Kawano, J., Oinuma, T., Hotta, K., Suganuma, T.: Fluid dynamics of the excretory flow of zymogenic and mucin contents in rat gastric gland processed by high pressure freezing/freeze substitution.
J Histochem Cytochem 50: 223-234, 2002
Sawaguchi, A., Tojo, H., Kawano, J., Okamoto, M., Suganuma, T.: Immunocytochemical demonstration of the secretory dynamics of zymogenic contents in rat gastric gland processed by high-pressure freezing/freeze substitution, with special references to phospholipase A2 and phospholipase Cg1.
Histochem Cell Biol. 116: 361-369, 2001
Kawano, J., Nakayama, T., Takami, Y., Kotani, T., Sawaguchi, A., Nagaike, R., Oinuma, T., Suganuma, T.: A monoclonal antibody against insect CALNUC recognizes the prooncoprotein EWS specifically in mammalian cells. Immunohistochemical and biolochemical studies of the antigen in rat tissues.
Histochem Cell Biol. 115: 421-428, 2001
Sawaguchi, A., Ide, S., Goto, Y., Kawano, J., Oinuma, T., Suganuma, T.: A simple contrast enhancement by potassium permanganate oxidation for Lowicryl K4M ultrathin sections prepared by high pressure freezing/freeze substitution.
J Microsc 201: 77-83, 2001
Date, Y., Kojima, M., Hosoda, H., Sawaguchi, A., Mondal, M.S., Suganuma, T., Matsukura, S., Kangawa, K., Nakazato, M.: Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans.
Endocrinology 141: 4255-4261, 2000
Kawano, J., Kotani, T., Ohtaki, S., Takechi, S., Nakayama, T., Sawaguchi, A., Nagaike, R., Oinuma, T., Suganuma, T.: CALNUC (nucleobindin) is located in the Golgi apparatus in insect cells.
Eur. J. Cell Biol. 79: 208-217, 2000
Sawaguchi, A., Ide, S., Kawano, J., Nagaike, R., Oinuma, T., Tojo, H., Okamoto, M., Suganuma, T.: Reappraisal of potassium permanganate oxidation applied to Lowicryl K4M embedded tissues processed by high pressure freezing / freeze substitution, with special reference to differential staining of the zymogen granules of rat gastric chief cells.
Arch. Histol. Cytol. 62: 447-458, 1999
Sawaguchi, A., Idate, Y., Ide, S., Kawano, J., Nagaike, R., Oinuma, T., Suganuma, T.: Multistratified expression of polysialic acid and its relationship to VAChT-containing neurons in the inner plexiform layer of adult rat retina.
J. Histochem. Cytochem. 47: 919-928, 1999
【和文総説】
髙橋伸育、豊嶋典世、澤口 朗：樹脂包埋補助ツールを用いたピンポイント電顕試料作
製法
顕微鏡48(2): 113-117, 2013
澤口 朗：超微形態と生体物質の保存に優れた加圧凍結技法
Plant Morphology 25: 7-10, 2013
澤口 朗：医学教育改革と解剖学教育の新たな潮流
宮崎県医師会医学会誌 37: 6-11, 2013
澤口 朗、豊嶋（青山） 典世、日野真一郎、髙橋 伸育：
理学療法士の技術力向上を目的とした卒後解剖学標本示説研修の新たな展開
理学療法学 38(8): 576-577, 2011
澤口 朗、豊嶋 典世：高圧凍結技法による胃粘膜の超微形態研究
顕微鏡 45: 130-132, 2010
澤口 朗、菅沼龍夫：高圧凍結技法の新展開─培養細胞への応用
顕微鏡 403: 204-206, 2005
菅沼龍夫、澤口 朗：凍結技法における新展開─高圧凍結技法
細 胞 35: 32-35, 2003
菅沼龍夫、澤口 朗、津山新一郎：高圧凍結・凍結置換法による形態と組織化学
電子顕微鏡 36: 99-101, 2001
